Nope, the smallest increment marked on the syringe is .5 of a unit. But it is quite easy to 'eyeball' a .25 of a unit, or even smaller increments. ...There is a photo of small doses somewhere, I'll try to find that for you. Another advantage of syringes is that you can make a dose 'fatter' or 'skinnier'. Using syringes means you can shave a bit of a unit if necessary, or make a unit 'fatter'. There is very much more flexibility than there is with the Vetpen. If a kitty needs small doses it's also worth considering using syringes made for U100 insulin (rather than those made for U40 insulin); but these have to be used in conjunction with our 'conversion chart' so that you know how much to draw up into the syringe to get the dose that you want. U100 syringes can make it a lot easier to measure very small doses. But you would need to be comfortable using the conversion chart.